@article{Hao_Yang_Yan_Li_Zhang_Yu_Zhang_Zhang_2017, title={Differences in expression of serum protein in patients with psoriasis vulgaris and blood heat syndrome and healthy volunteers}, volume={63}, url={https://www.cellmolbiol.org/index.php/CMB/article/view/1237}, DOI={10.14715/cmb/2017.63.8.9}, abstractNote={<p align="justify">To explore the mechanism of psoriasis vulgaris (PV), serum protein expression profiles between PV patients with blood-heat syndrome and healthy volunteers were detected by isobaric tags for relative and absolute quantitation (iTRAQ). First, sera from 15 PV patients with blood-heat syndrome and 10 healthy volunteers were collected; then, serum proteins were separated and hydrolyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a specific iTRAQ marker enzyme respectively after further purification and protein abundance treatment. Compared with the control group, differentially expressed proteins in PV patients with blood-heat syndrome were identified and analyzed by tandem mass spectrometry. A total of 787 proteins were identified and 718 proteins had a functional annotation with gene ontology (GO) by iTRAQ in the current study. Significant differences (P <0.05) and great differences (P <0.01) were found in 681 proteins and 536 proteins respectively between the patient group and healthy group. ). Different protein expression profiles in serum existed between PV patients with blood-heat syndrome and healthy volunteers; the differences largely involved immune-related proteins and lipoproteins. The proteins specific for PV with blood-heat syndrome deserves further investigation.</p>}, number={8}, journal={Cellular and Molecular Biology}, author={Hao, P. S. and Yang, C. and Yan, X. P. and Li, T. H. and Zhang, J. and Yu, Q. Y. and Zhang, X. and Zhang, Y. H.}, year={2017}, month={Aug.}, pages={38–41} }