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Identification of tumor stem-like cells in a mouse myeloma cell line
Corresponding Author(s) : J Dou
njdoujun@yahoo.com.cn
Cellular and Molecular Biology,
Vol. 55 No. 4: General Papers
Abstract
We used colony formation assay in the soft agar media or the serum-free media, the methods of identifying BrdU-label-retaining cells and the SP cells as well as the tumorigenicity test in BALB/c mice, respectively, to analyze tumor stem like cells in the SP2/0 cell line. The results showed that a few SP2/0 cells were capable of forming colonies in the soft agar media, contained BrdU-label-immortal strand in the SP2/0 cell line. The SP2/0 cells in the serum-free media gained higher tumorigenicity in the BALB/c mice than the SP2/0 cells cultivated in the complete media did. Overall, only a few of the SP2/0 cells were found to possess the characteristics of tumor stem-like cells, such as high proliferative potency, more self-renewal and stronger tumorigenesis, or greater similarity to the tumor stem cells (TSCs) traits. The biology of tumor stem-like cells contributes to the identification of molecular targets important for future tumor therapy.
Keywords
Tumor stem like cells
Tumor stem cells
Side population cells
Identification
Mouse myeloma cell line.
Dou, J., Li, Y., Zhao, F., Hu, W., Wen, P., Tang, Q., Chu, L., Wang, Y., Cao, M., Jiang, C., & Gu, N. (2009). Identification of tumor stem-like cells in a mouse myeloma cell line. Cellular and Molecular Biology, 55(4), 1151–60. Retrieved from https://www.cellmolbiol.org/index.php/CMB/article/view/1057
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