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The regulatory effects of autophagy to the CNE2 cells radio-sensitization
Corresponding Author(s) : G-L. Liu
liuguolongsy@126.com
Cellular and Molecular Biology,
Vol. 62 No. 13: Issue 13
Abstract
To investigate the effects of autophagy activator and autophagy inhibitor on the CNE2 radiation sensitivity of nasopharyngeal carcinoma cells. RNA interference technology was used to silence the atg5 gene and autophagy inhibition cell model was constructed. Rapamycin and chloroquine were treated respectively on cells with X-ray 5Gy irradiation. Cells' growth status were observed for 8 days and control group was set. The cell viability was detected by MTT assay and colony formation assay, and the cell cycle was analyzed by flow cytometry. Compared with the control group, the survival rate, clone formation rate and the survival rate of the irradiation of the other three groups were significantly lower. (P<0.05) Most cells were detected in the G0/G1 phase in the other three groups except the control group, and cells of the other two periods were less than those in the G0/G1 phase. The autophagy inhibitor or activator and atg5 silencing can be increased by CNE2 radiation therapy, however, the sensitization effect increase of autophagy activator is better than others.
Keywords
Autophagy
nasopharyngeal carcinoma
radiotherapy sensitization
rapamycin
chloroquine.
Li, B.-X., Cao, X.-F., Weng, C.-Y., Fang, X.-S., Mao, H.-B., Guan, M.-M., & Liu, G.-L. (2016). The regulatory effects of autophagy to the CNE2 cells radio-sensitization. Cellular and Molecular Biology, 62(13), 12–14. https://doi.org/10.14715/cmb/2016.62.13.3
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