The Na, K-ATPase alpha3-isoform specifically localizes in the Schmidt-Lanterman incisures of human nerve
Corresponding Author(s) : J.M. Maixent
Cellular and Molecular Biology,
Vol. 53 No. 6: General Papers
To our knowledge, there is little reference in literature with regards to alpha3-isoform of Na+,K+-ATPase in human peripheral nerves. The aim of this study was to determine the expression of the neuronal alpha3-isoform of Na+,K+-ATPase in human sural nerves from patients with a permanent medullary central nervous system injury. We studied the immunolocalization of alpha3-isoform of Na+,K+-ATPase using a polyclonal antibody against the amino sequence near the phosphorylation site of the alpha3-isoforms of Na+,K+-ATPase using immunohistochemistry and confocal laser scanning microscopy. An antibody specific for alpha2-isoform of Na+,K+-ATPase was used to label the Schwann cells. Morphometric analysis of longitudinal section of human sural nerves showed that the alpha3-isoform of Na+,K+-ATPase was distributed along the length of axolemma. The myelin sheath of the Schwann cells showed clearly a distribution of alpha3- but not alpha2-isoforms of Na+,K+-ATPase at the level of Schmidt-Lanterman incisures. The human sural nerve shows a specific localization of the Na+,K+-ATPase alpha3-isoform in the Schmidt-Lanterman incisures of Schwann cells in addition to its localization in axonal membranes.
Na K -ATPase Isoforms Immunohistochemistry Confocal laser scanning microscopy Central nervous system injury Peripheral nerve Schmidt-Lanterman clefts.
Rigoard, P., Tartarin, F., Buffenoir, K., Chaillou, M., Fares, M., D’Houtaud, S., Wager, M., Giot, J., Quellard, N., Fernandez, B., Lapierre, F., & Maixent, J. (2007). The Na, K-ATPase alpha3-isoform specifically localizes in the Schmidt-Lanterman incisures of human nerve. Cellular and Molecular Biology, 53(6), 1003–1009. Retrieved from https://www.cellmolbiol.org/index.php/CMB/article/view/1873
Download CitationEndnote/Zotero/Mendeley (RIS)