The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Role of free living, immobilized and non-viable biomass of Nostoc muscorum in removal of heavy metals: An impact of physiological state of biosorbent
Corresponding Author(s) : D. P. Singh
dpsingh_lko@yahoo.com
Cellular and Molecular Biology,
Vol. 60 No. 5: Bioremediation, bioenergy and stress biology
Abstract
Biosorption of Pb and Cd by using free living, immobilized living and non-viable forms of Nostocmuscorum was studied as a function of pH (3-8), contact time (5-240 min) and metal concentration (10-100 µg ml-1), to find out the most efficient physiological formfor metal removal. Results revealed that optimum conditions for biosorption of both the metals by different states of biosorbentwere almost same (contact time- 30 min, metal concentration- 100 µg ml-1 and pH- 5.1 and 6, for Pb and Cd, respectively) however, the immobilized biomass of N. muscorum was found to be more suitable for the development of an efficient biosorbent as evident from theqmax(1000 mg g-1protein) and Kf (0.08 mg g-1protein) values obtained from the Langmuir and Freundlich isotherms. A pseudo second order kinetics was found more suitable for describing the nature of biosorption of both the metals by all the three forms of N. muscorum. An analysis of correlation revealed that as the metal concentration increases, the removal of Pb and Cd by N. muscorum also increases significantly. The regression analysis showed that the rate of removal of Pb by free living and dead biomass was 1.89 and 1.58 times higher than the rate of removal of Cd by respective biomass. In contrast, the rate of removal of Cd by immobilized biomass was 1.46 times higher than that of Pb.
Keywords
Alginate beads
Immobilized
Isotherm
Kinetics
Nostoc muscorum.
Dixit, S., & Singh, D. P. (2014). Role of free living, immobilized and non-viable biomass of Nostoc muscorum in removal of heavy metals: An impact of physiological state of biosorbent. Cellular and Molecular Biology, 60(5), 110–118. Retrieved from https://www.cellmolbiol.org/index.php/CMB/article/view/509
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX