The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
rmpM gene as a genetic marker for human bacterial meningitis
Corresponding Author(s) : A. Kumar
ashokigib@rediffmail.com
Cellular and Molecular Biology,
Vol. 58 No. 1: Frontiers in biological sciences issue
Abstract
Meningitis is a bacterial, viral or fungal infection of the protective membrane meninges covering the brain and spinal cord. Viral and other forms of meningitis are mild and get cured within one or two week without any treatment. Whereas, bacterial meningitis can prove lethal if not being diagnosed or treated in time. Meningitis is a contagious infection and can spread from one person to another through coughing, sneezing or close contact. Usually the disease is diagnosed from cerebrospinal fluid (CSF) of the patients using culture, PCR, immunological and biochemical tests. All these methods suffer from one or more limitations. Our lab has developed a quick PCR based detection of Neisseria meningitidis (bacterial meningitis) directly from the patient CSF samples using specific primers of virulent rmpM gene. The overall analysis completes in 80 min for confirmation of the disease. Amplicon of 308 bp of rmpM gene does not show homology with other organisms and can be used as a genetic marker for human bacterial meningitis caused by Neisseria meningitidis.
Keywords
Bacterial meningitis
Genetic marker
N. meningitidis
rmpM gene.
Dash, S. K., Sharma, M., Khare, S., & Kumar, A. (2012). rmpM gene as a genetic marker for human bacterial meningitis. Cellular and Molecular Biology, 58(1), 26–30. Retrieved from https://www.cellmolbiol.org/index.php/CMB/article/view/575
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX