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Copyright (c) 2025 Rabia Ali Aboud, Safaa A. Fadil, Ahmed Akil Khudhair Al-Daoody, Amani M. Jasim
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Experimental visceral leishmaniasis: immunopathology and histology
Corresponding Author(s) : Amani M. Jasim
Cellular and Molecular Biology,
Vol. 71 No. 4: Issue 4
Abstract
This study aimed to assess the immunological and histopathological effects of Leishmania donovani infection in mice, and the impact of pentostam treatment. L. donovani promastigotes were cultured in Nicolle-Novy-McNeal (NNN) medium. Thirty mice were divided into three groups of ten: a negative control group given saline, a positive control group infected with promastigotes, and a treatment group infected with promastigotes and treated with pentostam. The mice were treated daily for 21 days. Blood samples were collected after 7, 14, and 21 days to measure serum levels of IL-1. After 21 days, the mice were euthanized, and their livers and spleens were collected for histopathological analysis. The results showed a significant decrease in IL-1 levels in the infected group compared to the control group, while IL-1 levels increased slightly in the treated group. Histopathological analysis revealed pathological changes in the liver and spleen of infected mice, which were reduced in the treated group. The study concluded that L. donovani infection leads to a decrease in IL-1 production and causes pathological damage to the liver and spleen, and that pentostam treatment is effective in mitigating these effects.
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