MicroRNA-92a Regulates Expression of Kruppel-like Factor2 in Rabbit Model of Intracranial Aneurysm
Corresponding Author(s) : X Wu
Cellular and Molecular Biology,
Vol. 61 No. 8: Issue 8
To explore role of miRNA-92a in regulation of KLF2 expression in intracranial aneurysm model, real time PCR, IHC, ISH and luciferase activity were used to test relationship of miR-92a and KLF2. The results demonstrated that KLF2 showed a time-dependent increase following development of IA model, but the miR-92a showed a time-dependent decrease, co-expression of miR-92a was found to effectively down-regulate luciferase expression in constructs with 3"²UTRs of KLF2, to determine the functions of miR-92a in rabbit ECs, we transfected the pre-miR-92a into rabbit ECs for over-expression, the results showed that the protein level of KLF2 was significantly down-regulated when over-expression of miR-92a. In conclusion, our research demonstrated that miR-92a and KLF2 were negative correlation in intracranial aneurysm model, and miR-92a could directly target KLF2 in endothelial cells through complementary sequence of 3"²UTR region.
MiRNA-92a target gene Kruppel-like Factor 2 intracranial aneurysm rabbit model.
Wu, X., Zhang, J., Huang, Q., Yang, P., Chen, J., & Liu, J. (2015). MicroRNA-92a Regulates Expression of Kruppel-like Factor2 in Rabbit Model of Intracranial Aneurysm. Cellular and Molecular Biology, 61(8), 44–48. Retrieved from https://www.cellmolbiol.org/index.php/CMB/article/view/756
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